Journal
TALANTA
Volume 215, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.talanta.2020.120892
Keywords
Fluorescent probe; Near-infrared (NIR); Hypochlorite; Live cell imaging
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Funding
- Science and Technology Developing Foundation of Jilin Province of China [20180201050YY]
- Industrial Innovation Funds of Jilin Province of China [2018C034-1]
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Highly sensitive specific and ultrasensitive fluorescent probes are employed for tracking ClO- for revealing its various cellular functions in living cells. In this work, a near-infrared fluorescence probe, XS-1, has been developed for tracking ClO-. The experimental results demonstrated that the probe, XS-1, could determine ClO- at the linear range of 0-2 mu M with a low detection limit of 72 nM (lambda(ex/em) = 580/626 nm). XS-1 can specifically detect ClO-, which can be applied for determining the basal ClO(- )and the fluctuation of exogenous ClO- in living cells. For further confirming its practicability, the probe was applied for detecting the hypochlorite content in the actual water sample. The recovery rate (92.7%-102.9%) displayed satisfactory values in the tap water and 84 disinfectants. In addition, we carried out theoretical calculations for clarifying the luminescence mechanism of the system.
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