Journal
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
Volume 233, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.saa.2020.118170
Keywords
Circular dichroism; Docking; Fludarabine; Fluorescence spectroscopy; HSA
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Funding
- National Natural Science Foundation of China [21873075, 21503283]
- Natural Science Foundation of Hubei Province [2015CFC873, 2018CFB135]
- Foundation of Ministry of Science and Technology of the People's Republic of China [BZY19022]
- Fundamental Research Funds for South-Central University for Nationalities [CZY20015]
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Fludarabine (Flu) is widely used to treat B-cell chronic lymphocytic leukemia. HSA is of the essence to human, especially in blood circulation system. The interaction mechanism between Flu and HSA was studied by comprehensive spectroscopic methods and molecular docking technique. UV-vis and FL spectrum results indicated that Flu bond with HSA, and there was a new complex produced at the binding site! in subdomain 11A. Association constants at 298 K were 1.637 x 10(4) M-1 and 1.552 x 10(4) M-1 at 310 K, respectively. The negative enthalpy (Delta H) and positive entropy (Delta S) values for the interaction revealed that the binding behavior was driven by hydrophobic forces and hydrogen bonds. The results obtained from UV, RLS spectra, 3D fluorescence and CD spectrum illustrated that Flu could change the secondary structure of HSA. According to molecule docking result, the binding energy of interaction is -11.15 kcal/mol. (C) 2020 Elsevier B.V. All rights reserved.
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