4.4 Article

Nonionic surfactant-based cloud point extraction of polyhydroxyalkanoate from the fermentation crude in a rotating disc contactor

Journal

SEPARATION SCIENCE AND TECHNOLOGY
Volume 56, Issue 9, Pages 1534-1545

Publisher

TAYLOR & FRANCIS INC
DOI: 10.1080/01496395.2020.1781895

Keywords

Cloud point extraction; polyhydroxyalkanoate; rotating disc contactor; Cupriavidus necator

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The nonionic surfactant-based cloud point extraction method was successfully implemented in a modified device to extract polyhydroxyalkanoate from fermentation broth. By studying the effect of rotor speed and phase flowrates, higher recovery rate and purity were achieved, while a correlation model was developed for predicting dispersed-phase holdup.
Nonionic surfactant-based cloud point extraction of polyhydroxyalkanoate from fermentation broth was implemented in a modified rotating disc contactor. The effect of rotor speed and phase flowrates on dispersed micellar phase holdup, mass transfer coefficient, yield, and purity were studied with micellar system contains TX114 (4.5 wt %)+ TMN6 (0.5 wt %) and 0.1 M ammonium chloride at broth pH of 3. The rotor speed and the micellar phase flowrate had a significant effect. A maximum PHA recovery of 85.48% and purity of 86.01% were achieved. The correlation was developed to predict the dispersed-phase holdup.

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