Determination of the response characteristics of anaerobic ammonium oxidation bioreactor disturbed by temperature change with the spectral fingerprint

Anaerobic ammonium oxidation (anammox) bacteria are sensitive and susceptible to operating condition fluctuations that can lead to the instability of a bioreactor. Through multivariate spectral analysis, the dynamic changes of intracellular and extracellular metabolites of anammox sludge under the declined temperature stress were characterized. It was found that effluent fluorescence components were positively related to the bacterial activity, and the response of the protein-like substances to the temperature change was more sensitive than that of humic substances. Under the transient disturbance during temperature change from 35 to 15 degrees C, anammox system tended to considerably excrete extracellular polymeric substances to resist the low temperature inhibition. However, the long-term exposure of the sludge at 10 degrees C resulted in the considerably inhibition of sludge activity, granular disintegration and heterotrophic denitrification bacteria increase. The two-dimensional correlation analysis further revealed that the humic add in extracellular polymeric substances was preferentially responded to the temperature change than protein. Anammox bacteria tended to increase the intracellular protein and electron transfer-related reactive substance excretion to counteract the low temperature inhibition. Herein, both the intra- and extracellular response characteristics of anammox sludge to temperature variation were successfully resolved via the combined spectra. This work provides a comprehensive understanding on the mechanism of anammox sludge to temperature variation and may be valuable for the development of bioreactor monitoring techniques. (C) 2020 Elsevier B.V. All rights reserved.