Barcoded microbial system for high-resolution object provenance

Determining where an object has been is a fundamental challenge for human health, commerce, and food safety. Location-specific microbes in principle offer a cheap and sensitive way to determine object provenance. We created a synthetic, scalable microbial spore system that identifies object provenance in under 1 hour at meter-scale resolution and near single-spore sensitivity and can be safely introduced into and recovered from the environment. This system solves the key challenges in object provenance: persistence in the environment scalability, rapid and facile decoding, and biocontainment Our system is compatible with SHERLOCK, a Cas13a RNA-guided nucleic acid detection assay, facilitating it implementation in a wide range of applications.