Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 117, Issue 33, Pages 20325-20333Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.2002115117
Keywords
snRNA; transcript termination; DSP1; alternative splicing; DSP1 alpha
Categories
Funding
- National Natural Science Foundation of China [31970603, 31971920]
- Natural Science Foundation of Guangxi province [2018JJG130001, 2018JJD130059]
- NIH [GM127414]
- NSF [MCB-1808182]
Ask authors/readers for more resources
Small nuclear RNAs (snRNAs) are the basal components of the spliceosome and play crucial roles in splicing. Their biogenesis is spatiotemporally regulated. However, related mechanisms are still poorly understood. Defective in snRNA processing (DSP1) is an essential component of the DSP1 complex that catalyzes plant snRNA 3'-end maturation by cotranscriptional endonucleolytic cleavage of the primary snRNA transcripts (presnRNAs). Here, we show that DSP1 is subjected to alternative splicing in pollens and embryos, resulting in two splicing variants, DSP1 alpha and DSP113. Un-like DSP1 alpha, DSP113 is not required for presnRNA 3'-end cleavage. Rather, it competes with DSP1 alpha for the interaction with CPSF73-I, the catalytic subunit of the DSP1 complex, which promotes effi-cient release of CPSF73-I and the DNA-dependent RNA polymer -ease II (Pol II) from the 3' end of snRNA loci thereby facilitates snRNA transcription termination, resulting in increased snRNA levels in pollens. Taken together, this study uncovers a mechanism that spatially regulates snRNA accumulation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available