Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 151, Issue -, Pages 157-165Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2020.03.001
Keywords
Abiotic stress; Betula platyphylla; BZR transcription factor; Gene expression; Hormone treatment
Categories
Funding
- Fundamental Research Funds for the Central Universities [2572019CG01]
- Province in Heilongjiang Outstanding Youth Science Fund [JC2017004]
- Heilongjiang Touyan Innovation Team Program (Tree Genetics and Breeding Innovation Team)
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Brassinazole-resistant (BZR) transcription factors have important roles in the brassinosteroid (BR) signalling pathway and are widely involved in plant growth and abiotic stress processes. However, there are few studies on the functions and regulatory mechanisms of BZR TFs in birch. In this study, 5 BZR genes were identified from birch. The qRT-PCR results showed that the expression levels of most BpBZRs were significantly downregulated and/or upregulated in at least one organ following NaCl and PEG stress or ABA, GA(3) and JA treatments. In particular, BpBZR1 expression was changed in all three organs after exposure to NaCl stress at all time points, indicating that this gene may be involved in salt stress. The BpBZR1 transcription factor was shown to have transcriptional activation activity in a yeast two-hybrid assay. Through a transient transformation system, we found that overexpression of BpBZR1 in birch resulted in lower H2O2 and MDA accumulation, higher SOD and POD activities and maintained a higher photosynthetic intensity and a lower chlorophyll degradation rate than those of the control plants under salt stress. These results preliminarily showed that overexpression of the BpBZR1 gene increased the tolerance of birch to salt stress.
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