Journal
PLANT JOURNAL
Volume 103, Issue 6, Pages 2263-2278Publisher
WILEY
DOI: 10.1111/tpj.14898
Keywords
protein-protein interaction; Solanum tuberosum(potato); TERMINAL FLOWER 1; CENTRORADIALIS; tuberigen complex; tuberisation
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Funding
- Scottish Government, Rural and Environment Science and Analytical Services Division
- GCRF Foundation Awards for Global Agricultural and Food Systems Research - BBSRC [BB/P022553/1]
- European Union [835704]
- Spanish Ministerio de Economia y Competitividad [BIO2015-73019-EXP]
- aligned Japan EIG CONCERT [PIA102017-1]
- Marie Curie Actions (MSCA) [835704] Funding Source: Marie Curie Actions (MSCA)
- BBSRC [BB/P022553/1, BB/M004899/1] Funding Source: UKRI
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Potato tuber formation is a secondary developmental programme by which cells in the subapical stolon region divide and radially expand to further differentiate into starch-accumulating parenchyma. Although some details of the molecular pathway that signals tuberisation are known, important gaps in our knowledge persist. Here, the role of a member of theTERMINAL FLOWER 1/CENTRORADIALISgene family (termedStCEN) in the negative control of tuberisation is demonstrated for what is thought to be the first time. It is shown that reduced expression ofStCENaccelerates tuber formation whereas transgenic lines overexpressing this gene display delayed tuberisation and reduced tuber yield. Protein-protein interaction studies (yeast two-hybrid and bimolecular fluorescence complementation) demonstrate that StCEN binds components of the recently described tuberigen activation complex. Using transient transactivation assays, we show that theStSP6Atuberisation signal is an activation target of the tuberigen activation complex, and that co-expression ofStCENblocks activation of theStSP6Agene by StFD-Like-1. Transcriptomic analysis of transgenic lines misexpressingStCENidentifies early transcriptional events in tuber formation. These results demonstrate that StCEN suppresses tuberisation by directly antagonising the function of StSP6A in stolons, identifyingStCENas a breeding marker to improve tuber initiation and yield through the selection of genotypes with reducedStCENexpression.
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