Cytokine and microbial profiles in relation to the clinical outcome following treatment of peri-implantitis

AimTo study whether cytokine levels and bacterial counts in p atients with peri-implantitis reflect clinical treatment outcome following non-surgical management. Materials and MethodsLuminex magnet bead technology and checkerboard DNA-DNA hybridization were used to assess treatment outcome after treatment at the implant with the most severe peri-implantitis in 41 participants. ResultsStudy group mean age was 40.3years (SD9.9). Stable treatment outcome after 6months (no further bone loss, probing pocket depth decrease 0.5mm, no bleeding/suppuration) was identified in 9 of 41 (22%) participants. Peri-implant crevicular fluid (PICF) levels were also lower for Il-1 (P<0.01), and with trends of lower cytokine levels in PICF for TNF- (P=0.071), PDGFBB (P=0.071), as well as for VEGF (vascular endothelial growth factor) (P=0.071), and bacterial counts for Actinomyces israelii, Aggregatibacter actonomycetemcomitans (Y4), Campylobacter gracilis, Echerichia coli, Fusobacterium periodonticum, Leptotrichia buccalis, Parvimonas micra, Staphylococcus haemolyticus, Streptococcus anginosus, and Tannerella forsythia. Increasing levels of IL-1 and S.aureus (r(2)=0.856) were found only at implants with non-stable outcome. A reduction of PICF levels for selected cytokines and bacteria studied had a sensitivity of 0.77, and a specificity of 0.80 against the clinical outcome as gold standard. Data analysis failed to differences in treatments (PerioFlow((R)) versus YAG: ER laser) for changes in the expression of cytokines and bacteria studied. ConclusionsAt 6months, clinically stable treatment outcome of peri-implantitis is associated lower levels of putative pathogens total bacterial load with 30% reduction of IL1-, L-6, and VEGF levels in PICF.