Journal
ORGANIC PROCESS RESEARCH & DEVELOPMENT
Volume 24, Issue 8, Pages 1467-1476Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.oprd.0c00200
Keywords
GABA(A) receptor; asthma; imidazodiazepine; amino acid N-carboxyanhydride
Categories
Funding
- National Institutes of Health (USA) [R41HL147658, R01NS076517, R01HL118561]
- University of Wisconsin-Milwaukee
- University of Wisconsin-Milwaukee Research Foundation (Catalyst grant)
- Lynde and Harry Bradley Foundation
- Richard and Ethel Herzfeld Foundation
- National Science Foundation, Division of Chemistry [CHE-1625735]
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We report an improved and scalable synthesis of MIDD0301, a positive GABA(A) receptor modulator that is under development as oral and inhaled treatments for asthma. In contrast to other benzodiazepines in clinical use, MIDD0301 is a chiral compound that has limited brain absorption. The starting material to generate MIDD0301 is 2-amino-5-bromo-2'-fluorobenzophenone, which has a nonbasic nitrogen due to electron-withdrawing substituents in the ortho and para positions, reducing its reactivity toward activated carboxylic acids. Investigations of peptide coupling reagents on a multigram scale resulted in moderate yields due to incomplete conversions. Second, the basic conditions used for the formation of the seven-membered 1,4-diazepine ring resulted in racemization of the chiral center. We found that neutral conditions comparable to the pK a of the primary amine were sufficient to support the formation of the intramolecular imine but did not enable the simultaneous removal of the protecting group. Both difficulties were overcome with the application of the N-carboxyanhydride of u-alanine. Activated in the presence of an acid, this compound reacted with nonbasic 2-amino-5-bromo-2'-fluorobenzophenone and formed the 1,4-diazepine upon neutralization with triethylamine. Carefully designed workup procedures and divergent solubility of the synthesic intermediates in solvents and solvent combinations were utilized to eliminate the need for column chromatography. To improve compatibility with large-scale reactors, temperature-controlled slow addition of reagents generated the imidazodiazepine at -20 degrees C. All intermediates were isolated with a purity of >97% and impurities were identified and quantified. After the final hydrolysis step, MIDD0301 was isolated in a 44% overall yield and a purity of 98.9% after recrystallization. The enantiomeric excess was greater than 99.0%.
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