Journal
NUCLEIC ACIDS RESEARCH
Volume 48, Issue 15, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkaa521
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Funding
- National Human Genome Research Institute [R01HG008752]
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Understanding the thermodynamics of DNA motifs is important for prediction and design of probes and primers, but melt curve analyses are low-throughput and produce inaccurate results for motifs such as bulges and mismatches. Here, we developed a new, accurate and high-throughput method for measuring DNA motif thermodynamics called TEEM (Toehold Exchange Energy Measurement). It is a refined framework of comparing two toehold exchange reactions, which are competitive strand displacement between oligonucleotides. In a single experiment, TEEM can measure over 1000 Delta G degrees. values with standard error of roughly 0.05 kcal/mol.
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