Journal
NEUROSCIENCE LETTERS
Volume 732, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.neulet.2020.135072
Keywords
Immediate-early gene; Transgenic mouse; Zif268; Activity-dependent; In vivo imaging; Reporter
Categories
Funding
- Alberta Innovates -Health Solutions Polaris
- Natural Sciences and Engineering Research Council of Canada (NSERC) [1631465]
- NSERC Canada Graduate Scholarship Doctoral (CGS-D3) award
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Transgenic immediate-early gene reporter mouse strains are valuable tools for studying activity-dependent neural cell populations in vivo. However, routine characterization of the Gene Expression Nervous System Atlas (GENSAT) Egr1-EGFP reporter mouse strain produced results that were highly inconsistent with endogenous Egr1 expression. Activity-dependent EGFP expression was not observed, and EGFP protein did not co-localize with native Egr1 protein. This precautionary study outlines the limitations of the Egr1-EGFP transgenic line as a tool to study the activity-dependent expression of Egr1 and emphasizes the necessity of taking into account the potential loss of regulatory elements, stability determinants, or translational modulation in transgenic reporter strains.
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