Journal
NEUROSCIENCE
Volume 436, Issue -, Pages 11-26Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2020.04.001
Keywords
PDGFR alpha; oligodendrocytes; hypomyelination; differentiation; survival; vascular abnormality
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology, Japan [JP25293093/JP17H04062, JP23590444, JP23122506/JP26460360]
Ask authors/readers for more resources
The platelet-derived growth factor receptor-alpha (PDGFR alpha) principally mediates growth factor signals in oligodendroglial progenitors and is involved in oligodendrogenesis and myelinogenesis in the developing spinal cord. However, the role of PDGFR alpha in the developing forebrain remains relatively unknown. We established a conditional knockout mouse for the Pdgfra gene (N-PR alpha-KO) using a Nestin promoter/enhancer-driven Cre recombinase and examined forebrain development. The expression of was efficiently suppressed in the Olig2(+) cells in N-PR alpha-KO mice. In these mice, Olig2(+) cells were slightly decreased during embryonic periods. The decrease was particularly striking during the postnatal period. The commitment of Pdgfra-inactivated Olig2(+) cells to Sox10(+) oligodendroglial-lineage was largely suppressed. Surviving Olig2(+) cells and Sox10(+) cells were distributed widely in the N-PR alpha-KO mouse brain, similarly to those in control mice until the early neonatal period. After that, these cells were drastically depleted in the forebrain during the second postnatal week. The brains of N-PR alpha-KO mice were severely hypomyelinated, and these mice died on approximately P17 with motor disturbances. Disturbed axonal fibers and extensively aberrant vascular formations appeared in the postnatal N-PR alpha-KO mouse brains. After the defective PDGFR alpha signal in the forebrain, these phenotypes were clearly different from those in the spinal cord that showed defective populations expansion and migration of oligodendroglial lineage and premature myelination, as previously described. In contrast, areas of severe hypomyelination were common to both anatomical sites. PDGFR alpha was critically involved in the myelination of the forebrain and may differently regulate oligodendroglial lineage between the forebrain and spinal cord. (C) 2020 IBRO. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available