Nanolipoidal α-terpineol modulates quorum sensing regulated virulence and biofilm formation in Pseudomonas aeruginosa

Aim:Pseudomonas aeruginosahas emerged as a major opportunistic pathogen meaning there is an immediate need to develop efficient antivirulence agents which offer a new class of superior therapeutics.Methods:Nanostructured lipid carriers (NLCs) containing alpha-terpineol (alpha T) were developed and characterized to determine expression profiles of quorum sensing regulated genes, antivirulence activity and antibiofilm effects againstP. aeruginosa.Results:The alpha T-NLCs had a size of 145.4 nm, polydispersity index of 0.242 and zeta-potential of -31.4 mV. They exhibited pronounced effects on the inhibition of quorum sensing mediated virulence and biofilm formation which were confirmed by molecular docking analysis and gene expression profiles.Conclusion:alpha T-NLCs show promise as effective antivirulence agents againstP. aeruginosain the postantibiotic era. [GRAPHICS] .