Journal
NANOMEDICINE
Volume 15, Issue 17, Pages 1687-1707Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/nnm-2020-0106
Keywords
brain tumor; conjugated polymer nanoparticles; monocytes-macrophages; photodynamic therapy; Trojan horse therapy
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (PIPCONICET), Argentina [11220150100295CO, 11220150100069]
- Agencia Nacional de Promocion Cientifica y Tecnologica (PICT), Argentina [2676/18, 3577/18, 914/14]
- Instituto Nacional del Cancer, Argentina [INC-2016]
- Ministerio de Ciencia y Tecnologia de la Prov. de Cordoba (MINCyT Cba), Argentina [GRFT/17, PIODO/18]
- Secretaria de Ciencia y Tecnica Universidad Nacional de Rio Cuarto (PPI-SECyT UNRC), Argentina
- Ministry of Economy, Industry and Competitivity, Spain [SAF2017-83043-R]
- Program MULTITARGET&VIEW-CM from Community of Madrid, Spain [S2017/BMD-3688]
- FEDER
- FSE funds
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Aim: To assess monocyte-based delivery of conjugated polymer nanoparticles (CPNs) for improved photodynamic therapy (PDT) in glioblastoma (GBM). Materials & methods: Human monocyte cells (THP-1) and murine monocytes isolated from bone marrow (mBMDMs) were employed as stealth CPN carriers to penetrate into GBM spheroids and an orthotopic model of the tumor. The success of PDT, using this cell-mediated targeting strategy, was determined by its effect on the spheroids. Results: CPNs did not affect monocyte viability in the absence of light and did not show nonspecific release after cell loading. Activated monocytes incorporated CPNs in a higher proportion than monocytes in their naive state, without a loss of cellular functionality. In vitro PDT efficacy using cell-mediated delivery was superior to that using non vehiculized CPNs. Conclusion: CPN-loaded monocytes could efficiently deliver CPNs into GBM spheroids and the orthotopic model. Improved PDT in spheroids was confirmed using this delivery strategy.
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