Journal
NANO LETTERS
Volume 20, Issue 9, Pages 6466-6472Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.0c02163
Keywords
Intracellular temperature mapping; Luminescence thermometry; Thermogenesis; Lanthanide-based polymer nanomicelles
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Funding
- Portuguese funds through the FCT/MEC [UIDB/50011/2020, UIDP/50011/2020]
- FEDER under the PT2020 Partnership Agreement
- FEDER, through POCI [POCI-01-0145-FEDER-031469]
- Portuguese funds (OE), through FCT/MCTES
- European Union [801305, 829162]
- Spanish Ministry of Science Innovation and Universities [PGC2018_095795_B_I00]
- Diputacion General de Aragon [E11/17R]
- SusPhotoSolutions project, Portugal [CENTRO-01-0145FEDER-000005]
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Measurement of thermogenesis in individual cells is a remarkable challenge due to the complexity of the biochemical environment (such as pH and ionic strength) and to the rapid and yet not well-understood heat transfer mechanisms throughout the cell. Here, we present a unique system for intracellular temperature mapping in a fluorescence microscope (uncertainty of 0.2 K) using rationally designed luminescent Ln(3+)-bearing polymeric micellar probes (Ln = Sm, Eu) incubated in breast cancer MDA-MB468 cells. Two-dimensional (2D) thermal images recorded increasing the temperature of the cells culture medium between 296 and 304 K shows inhomogeneous intracellular temperature progressions up to similar to 20 degrees and subcellular gradients of similar to 5 degrees between the nucleolus and the rest of the cell, illustrating the thermogenic activity of the different organelles and highlighting the potential of this tool to study intracellular processes.
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