Journal
MOLECULES
Volume 25, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/molecules25153478
Keywords
fractionation; reducing activity; biomolecules; antibiofilm; microbial infection
Funding
- Provincial Education Department Project (Natural Science) [2017KZDXM045]
- Guangzhou Foreign Cooperation Project [201907010033]
- Graduate Technology Innovation Fund [KJCX2019004]
- Undergraduate Innovation and Entrepreneurship Training Program [S201911347028]
- Islamia University Bahawalpur, Pakistan, National Research Program for University (NRPU) for Higher Education Commission [9458]
- Agriculture and Rural Department Project of Guangdong Province
Ask authors/readers for more resources
Withania coagulanscontains a complex mixture of various bioactive compounds. In order to reduce the complexity of the plant extract to purify its phytochemical biomolecules, a novel fractionation strategy using different solvent combination ratios was applied to isolate twelve bioactive fractions. These fractions were tested for activity in the biogenic synthesis of cobalt oxide nanoparticles, biofilm and antifungal activities. The results revealed that plant extract with bioactive fractions in 30% ratio for all solvent combinations showed more potent bioreducing power, according to the observed color changes and the appearance of representative absorption peaks at 500-510 nm in the UV-visible spectra which confirm the synthesis of cobalt oxide nanoparticles (Co(3)O(4)NPs). XRD diffraction was used to define the crystal structure, size and phase composition of the products. The fractions obtained using 90% methanol/hexane and 30% methanol/hexane showed more effectiveness against biofilm formation byPseudomonas aeruginosaandStaphylococcus aureusso these fractions could potentially be used to treat bacterial infections. The 90% hexane/H2O fraction showed excellent antifungal activity againstAspergillus nigerandCandida albicans, while the 70% methanol/hexane fraction showed good antifungal activity forC. albicans, so these fractions are potentially useful for the treatment of various fungal infections. On the whole it was concluded that fractionation based on effective combinations of methanol/hexane was useful to investigate and study bioactive compounds, and the active compounds from these fractions may be further purified and tested in various clinical trials.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available