Chronic administration of ketamine induces cognitive deterioration by restraining synaptic signaling

The discovery of the rapid antidepressant effects of ketamine has arguably been the most important advance in depression treatment. Recently, it was reported that repeated long-term ketamine administration is effective in preventing relapse of depression, which may broaden the clinical use of ketamine. However, long-term treatment with ketamine produces cognitive impairments, and the underlying molecular mechanisms for these impairments are largely unknown. Here, we found that chronic in vivo exposure to ketamine for 28 days led to decreased expression of the glutamate receptor subunits GluA1, GluA2, GluN2A, and GluN2B; decreased expression of the synaptic proteins Syn and PSD-95; decreased dendrite spine density; impairments in long-term potentiation (LTP) and synaptic transmission in the hippocampal CA1 area; and deterioration of learning and memory in mice. Furthermore, the reduced glutamate receptor subunit and synaptic protein expression and the LTP deficits were still observed on day 28 after the last injection of ketamine. We found that the expression and phosphorylation of CaMKII beta, ERK1/2, CREB, and NF-kappa B were inhibited by ketamine. The reductions in glutamate receptor subunit expression and dendritic spine density and the deficits in LTP, synaptic transmission, and cognition were alleviated by overexpression of CaMKII beta. Our study indicates that inhibition of CaMKII beta-ERK1/2-CREB/NF-kappa B signaling may mediate chronic ketamine use-associated cognitive impairments by restraining synaptic signaling. Hypofunction of the glutamatergic system might be the underlying mechanism accounting for chronic ketamine use-associated cognitive impairments. Our findings may suggest possible strategies to alleviate ketamine use-associated cognitive deficits and broaden the clinical use of ketamine in depression treatment.

Automated summary

The discovery of the rapid antidepressant effects of ketamine has been considered a significant advancement in depression treatment. However, long-term use of ketamine may lead to cognitive impairments, possibly due to reductions in glutamate receptor subunits and synaptic signaling. The study suggests that overexpression of CaMKIIβ could alleviate these impairments, while inhibition of CaMKIIβ-ERK1/2-CREB/NF-κB signaling may mediate the cognitive deficits associated with chronic ketamine use.