Journal
MOLECULAR & CELLULAR PROTEOMICS
Volume 20, Issue -, Pages -Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/mcp.R120.002095
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Funding
- Vilas Distinguished Achievement Professorship and Charles Melbourne Johnson Distinguished Chair Professorship
- Wisconsin Alumni Research Foundation
- University of Wisconsin-Madison School of Pharmacy
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This review discusses the growing importance of glycosylation in physiological events and human diseases, and presents various strategies and workflows for accurate quantification of glycans and glycopeptides, including mass spectrometry-based techniques and label-free quantification.
Growing implications of glycosylation in physiological occurrences and human disease have prompted intensive focus on revealing glycomic perturbations through absolute and relative quantification. Empowered by seminal methodologies and increasing capacity for detection, identification, and characterization, the past decade has provided a significant increase in the number of suitable strategies for glycan and glycopeptide quantification. Mass-spectrometry-based strategies for glycomic quantitation have grown to include metabolic incorporation of stable isotopes, deposition of mass difference and mass defect isotopic labels, and isobaric chemical labeling, providing researchers with ample tools for accurate and robust quantitation. Beyond this, workflows have been designed to harness instrument capability for label-free quantification, and numerous software packages have been developed to facilitate reliable spectrum scoring. In this review, we present and highlight the most recent advances in chemical labeling and associated techniques for glycan and glycopeptide quantification.
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