Silver molybdate nanoparticles based immunosensor for the non-invasive detection of Interleukin-8 biomarker

In this study, we report the silver molybdate nanoparticles (beta-Ag2MoO4 NPs) based non-invasive and sensitive electrochemical immunosensor for label-free detection of Interleukin-8 (IL-8) biomarker. The X-ray diffraction and Raman spectroscopy studies confirm the cubic spinel structures of beta-Ag2MoO4 NPs. High-resolution transmission electron microscopy study depicted average size of beta-Ag2MoO4 NPs as 27.15 nm. The cleaned indium tin oxide coated glass substrates were coated with spin-coated thin films of Ag2MoO4 NPs. These electrodes used for covalently immobilization of antibodies specific to IL-8 (Anti-IL-8) using EDC-NHS chemistry and unbound activated sites blocked by bovine serum albumin. Electrochemical response was obtained in the range of 1 mu g mL(-1) to 40 ng mL(-1) and the sensitivity was found to be 7.03 mu A ng(-1)mL cm(-2) with LOD of 90 pg mL(-1). Spiked samples prepared by human saliva were tested and found efficient detection with this immunoelectrode.