Genome-Inspired Chemical Exploration of Marine FungusAspergillus fumigatusMF071

The marine-derived fungusAspergillusfumigatusMF071, isolated from sediment collected from the Bohai Sea, China, yielded two new compounds 19S,20-epoxy-18-oxotryprostatin A (1) and 20-hydroxy-18-oxotryprostatin A (2), in addition to 28 known compounds (3-30). The chemical structures were established on the basis of 1D, 2D NMR and HRESIMS spectroscopic data. This is the first report on NMR data of monomethylsulochrin-4-sulphate (4) and pseurotin H (10) as naturally occurring compounds. Compounds15,16,20,23, and30displayed weak antibacterial activity (minimum inhibitory concentration: 100 mu g/mL). Compounds18and19exhibited strong activity againstS. aureus(minimum inhibitory concentration: 6.25 and 3.13 mu g/mL, respectively) andE. coli(minimum inhibitory concentration: 6.25 and 3.13 mu g/mL, respectively). A genomic data analysis revealed the putative biosynthetic gene clustersftmfor fumitremorgins,psofor pseurotins,fgafor fumigaclavines, andhelfor helvolinic acid. These putative biosynthetic gene clusters fundamentally underpinned the enzymatic and mechanistic function study for the biosynthesis of these compounds. The current study reported two new compounds and biosynthetic gene clusters of fumitremorgins, pseurotins, fumigaclavines and helvolinic acid fromAspergillusfumigatusMF071.