Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 130, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2020.109673
Keywords
Anthocyanin; Enzymatic acylation; Colour stability; Antioxidation activity
Categories
Funding
- Natural Science Foundation of Fujian Province of China [2019J05109, 2018J05060]
- Natural Science Foundation of Zhangzhou of China [ZZ2019J22]
- National Undergraduate Innovation and Entrepreneurship Funding Program of China [1040224, 1040276]
- Educational Reform Project of Minnan Normal University of China [JG201937]
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In this work, p-coumaric acid and caffeic acid were grafted on blueberry anthocyanins through enzyme catalysis method to improve their colour stability and antioxidant activity. The acylation degrees of p-coumaric acylated anthocyanins (Co-An) and caffeic acid anthocyanins (Ca-An) reached 5.38% and 5.68%, respectively. The results from Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, ultraviolet visible spectroscopy and hydrogen nuclear magnetic resonance implied that p-coumaric acid and caffeic acid grafted on the 6-OH of glucoside and galactoside and the 5-OH of arabinose through esterification reaction. As revealed in the in vitro experiment on antioxidation activity and the experiment on colour stability, Co-An and Ca-An showed stronger antioxidant activity in DPPH assay and beta-carotene bleaching assay and higher colour stability during storage at 25 degrees C, 40 degrees C and 60 degrees C than native blueberry anthocyanins.
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