Journal
LABORATORY ANIMALS
Volume 55, Issue 1, Pages 13-20Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0023677220928091
Keywords
Immunodeficient mice; NOG mice; inhibin antiserum; equine chorionic gonadotropin; superovulation; age
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Funding
- Central Institute for Experimental Animals (CIEA)
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Severe immunodeficient NOG mice were efficiently produced using the superovulation technique with eCG and IAS treatment, resulting in increased oocyte production and successful fertilization and development into blastocysts and pups across different ages.
Severe immunodeficient mice are an essential tool for the examination of the efficacy and safety of new therapeutic technologies as a humanized model. Previously, non-obese diabetic (NOD)/Shi-scid IL2r gamma(null) (NOG) mice were established as immunodeficient mice by combining interleukin-2 receptor-gamma chain-knockout mice and NOD/Shi-scid mice. The NOG mice are used frequently in the research of therapeutic monoclonal antibodies and regenerative medicine for human diseases. Establishment of an efficient production system of NOG mice, using optimized reproductive techniques, is required to accelerate research. In this study, we investigated the efficacy of the superovulation technique using equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS) in NOG mice of various ages (4, 8, 12, 24, or 54 weeks). Additionally, we examined the fertilizing and developmental ability of the oocytes through in-vitro fertilization using frozen-thawed sperm, embryo culture and embryo transfer. The results showed that NOG mice produced the highest number of oocytes at 12 weeks old following the co-administration of eCG and IAS (collectively IASe) (70 oocytes/female). IASe was more effective in increasing the number of oocytes v. eCG at all ages. The IASe-derived oocytes demonstrated the ability to fertilize and develop into blastocysts and pups. Finally, we demonstrated that three strains of genetically modified NOG mice were efficiently produced through the optimized reproductive techniques. In summary, we developed an efficient system for the production of immunodeficient mice using 12-week-old, IASe-treated female NOG mice.
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