Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 101, Issue 3, Pages 1030-1037Publisher
WILEY
DOI: 10.1002/jsfa.10711
Keywords
artificial gastric juice; molecular markers; poisonous plants; qPCR; SNPs
Funding
- Ministry of Food and Drug Safety [17162MFDS065]
Ask authors/readers for more resources
The developed species-specific primer sets were able to successfully detect target DNA in commercial food products and digested samples, serving as useful tools for distinguishing between poisonous plants and edible plants.
BACKGROUND As a result of similar appearances between edible and poisonous plants, 42 patients have ingested poisonous plants from 2013 to 2017 in Korea. We have developed species-specific primer sets of three of edible and poisonous plants sets (Ligularia fischeri&Caltha palustris,Artemisia annua & Ambrosia artemisiifoliaandHemerocallis fulva&Veratrum maackii) for distinguishing both plants using a real-time polymerase chain reaction assay. RESULTS The efficiencies of the developed primer sets ranged from 87.8% to 102.0%. The developed primer sets have significant correlation coefficient values between the Ct values and the log DNA concentration for their target species (r(2) > 0.99). The cut-off lines as the crossing point values of the limit of quantitation of the target species were determined, and all non-target species were amplified later than the cut-off cycles. Then, the effectiveness of the developed primer sets was evaluated using commercial food products and digested samples with simulated gastric juice. CONCLUSION All of the developed species-specific primer sets were able to detect target DNA successfully in commercial food products and the digested samples. Therefore, the developed species-specific primer sets in the present study would be useful tools for distinguishing between poisonous plants and edible plants. (c) 2020 Society of Chemical Industry
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available