Journal
JOURNAL OF THE ACOUSTICAL SOCIETY OF AMERICA
Volume 148, Issue 1, Pages EL58-EL64Publisher
ACOUSTICAL SOC AMER AMER INST PHYSICS
DOI: 10.1121/10.0001590
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- Ministry of Science and Technology, Taiwan
- MOST [107-2221-E-002-068-MY3, MOST107-2321-B-002-040, MOST 107-2321-B-010-012, 108-2321-B-002-061-MY2]
- National Health Research Institute, Taiwan [NHRI-EX109-10924EI]
- National Taiwan University [NTU-CC-107L891105]
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A cellular stimulation device utilizing an AT-cut quartz coverslip mounted on an ultrasonic live imaging chamber is developed to investigate the effect of piezoelectric stimulation. Two types of chambers deliver ultrasound at intensities ranging from 1 to 20 mW/cm(2) to mesenchymal stem cells (MSCs) seeded on the quartz coverslip. The quartz coverslip imposes additionally localized electric charges as it vibrates with the stimulation. The device was applied to explore whether piezoelectric stimulation can facilitate chondrogenesis of MSCs. The results suggest piezoelectric stimulation drove clustering of MSCs and consequently facilitated chondrogenesis of MSCs without the use of differentiation media. (C) 2020 Acoustical Society of America
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