Journal
JOURNAL OF PROTEOMICS
Volume 225, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2020.103883
Keywords
Metabolomics; 2-Hydroxybutyric acid; 2-Oxoglutaric acid; Pregnancy; Birth; Bovine
Categories
Funding
- Spanish Ministry of Economy and Competitiveness (MINECO) [AGL2016-78597R, AGL2016-81890-REDT]
- Fondo Europeo de Desarrollo Regional (FEDER)
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Blood biomarkers may help to predict pregnancy in recipients of in vitro produced (IVP) embryos. Using H-1 nuclear magnetic resonance, we quantified 36 metabolites in the blood plasma of recipients (90% heifers, healthy, 1.95 years on average at the time of 1st embryo transfer -ET-) collected at Day-0 (estrus) and Day-7 (before ET time). First, IVP embryos were transferred to Asturiana de los Valles recipients as fresh (F) (N=26) and vitrified/warmed (V/W) (N=48) (discovery groups). Only at estrus, we discovered 4, 11, and 5 (F-ET), and 2, 2, and 4 (V/W-ET) metabolites that predicted pregnancy on Day-40, Day-62 and calving time, respectively (ROC-AUC > 0.700; P<.05). Thereafter, validation was performed in independent samples (N=67 F and N=63 V/W) of three cattle breeds by an index of overall classification accuracy (OCA> 0.650, P<.05). The numbers of candidate biomarkers validated were 2, 9 and 1 (F-ET) and 2, 2, and 3 (V/W-ET) on Day 40, Day-62 and calving time. Relevant metabolites were validated at the three (2-Oxoglutaric acid (F-ET), and 2-Hydroxybutyric acid and Dimethylamine (V/W-ET)) and two pregnancy endpoints (Ketoleucine (F-ET); Day-40 and Day-62) analysed. Fatty acid degradation and oxidative metabolism were enriched in pregnant recipients. The candidate biomarkers identified can improve embryo-recipient selection. Significance: We identified, for the first time, reliable pregnancy and birth candidate metabolite biomarkers for fresh and vitrified IVP embryos in blood of beef cattle recipients. Our findings can help to improve embryo-recipient selection, which is usually carried out in a way that females that will not become pregnant are not well differentiated.
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