Journal
JOURNAL OF PHYSIOLOGY-LONDON
Volume 598, Issue 19, Pages 4209-4223Publisher
WILEY
DOI: 10.1113/JP279919
Keywords
alzheimer's disease; beta-amyloid; brain-gut axis
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Funding
- Chinese University of Hong Kong
- Emesis Group, the Chinese University of Hong Kong
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Key points Alzheimer's disease (AD) patients and transgenic mice have beta-amyloid (A beta) aggregation in the gastrointestinal (GI) tract. It is possible that A beta from the periphery contributes to the load of A beta in the brain, as A beta has prion-like properties. The present investigations demonstrate that A beta injected into the GI tract of ICR mice is internalised into enteric cholinergic neurons; at 1 month, administration of A beta into the body of the stomach and the proximal colon was observed to partly redistribute to the fundus and jejunum; at 1 year, vagal and cerebral beta-amyloidosis was present, and mice exhibited GI dysfunction and cognitive deficits. These data reveal a previously undiscovered mechanism that potentially contributes to the development of AD. Alzheimer's disease (AD) is the most common age-related cause of dementia, characterised by extracellular beta-amyloid (A beta) plaques and intracellular phosphorylated tau tangles in the brain. A beta deposits have also been observed in the gastrointestinal (GI) tract of AD patients and transgenic mice, with overexpression of amyloid precursor protein. In the present studies, we investigate whether intra-GI administration of A beta can potentially induce amyloidosis in the central nervous system (CNS) and AD-related pathology such as dementia. We micro-injected A beta 1-42 oligomers (4 mu g per site, five sites) or vehicle (saline, 5 mu l) into the gastric wall of ICR mice under general anaesthesia. Immunofluorescence staining andin vivoimaging showed that HiLyte Fluor 555-labelled A beta 1-42 had migrated within 3 h via the submucosa to nearby areas and was internalised into cholinergic neurons. At 1 month, HiLyte Fluor 555-labelled A beta 1-42 in the body of the stomach and proximal colon had partly re-distributed to the fundus and jejunum. At 1 year, the jejunum showed functional alterations in neuromuscular coupling (P < 0.001), and A beta deposits were present in the vagus and brain, with animals exhibiting cognitive impairments in the Y-maze spontaneous alteration test (P < 0.001) and the novel object recognition test (P < 0.001). We found that enteric A beta oligomers induce an alteration in gastric function, amyloidosis in the CNS, and AD-like dementia via vagal mechanisms. Our results suggest that A beta load is likely to occur initially in the GI tract and may translocate to the brain, opening the possibility of new strategies for the early diagnosis and prevention of AD.
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