Journal
JOURNAL OF ORAL IMPLANTOLOGY
Volume 47, Issue 2, Pages 93-101Publisher
ALLEN PRESS INC
DOI: 10.1563/aaid-joi-D-19-00148
Keywords
bone tissue engineering; hydroxyapatite; polymers; simvastatin
Categories
Funding
- International Team for Implantology Foundation [1113_ 2015]
Ask authors/readers for more resources
The study aimed to synthesize, characterize, and evaluate the biocompatibility of PLGA+HA/β-TCP scaffolds incorporating simvastatin for bone tissue engineering. The inclusion of simvastatin improved scaffold hydrophilicity and showed adequate structural, chemical, thermal, and biological properties for bone tissue engineering.
The aim of this study was to synthesize, characterize, and evaluate degradation and biocompatibility of poly(lactic-co-glycolic acid) thorn hydroxyapatite/beta-tricalcium phosphate (PLGA+HA/beta TCP) scaffolds incorporating simvastatin (SIM) to verify if this biomaterial might be promising for bone tissue engineering. Samples were obtained by the solvent evaporation technique. Biphasic ceramic particles (70% HA, 30% beta TCP) were added to PLGA in a ratio of 1:1. Samples with SIM received 1% (m/m) of this medication. Scaffolds were synthesized in a cylindric shape and sterilized by ethylene oxide. For degradation analysis, samples were immersed in phosphate-buffered saline at 378C under constant stirring for 7, 14, 21, and 28 days. Nondegraded samples were taken as reference. Mass variation, scanning electron microscopy, porosity analysis, Fourier transform infrared spectroscopy, differential scanning calorimetry, and thermogravimetry were performed to evaluate physico-chemical properties. Wettability and cytotoxicity tests were conducted to evaluate the biocompatibility. Microscopic images revealed the presence of macro-, meso-, and micropores in the polymer structure with HA/beta TCP particles homogeneously dispersed. Chemical and thermal analyses presented similar results for both PLGA+HA/beta TCP and PLGA+HA/beta TCPthornSIM. The incorporation of simvastatin improved the hydrophilicity of scaffolds. Additionally, PLGA+HA/beta TCP and PLGA+HA/beta TCPthornSIM scaffolds were biocompatible for osteoblasts and mesenchymal stem cells. In summary, PLGA+HA/beta TCP scaffolds incorporating simvastatin presented adequate structural, chemical, thermal, and biological properties for bone tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available