Journal
JOURNAL OF MOLECULAR ENDOCRINOLOGY
Volume 65, Issue 2, Pages R35-R51Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/JME-19-0276
Keywords
technology; single cell; microfluidics; multi-omits; transcriptome; endocrine organs
Categories
Funding
- Francis Crick Institute
- Cancer Research UK [FC001107]
- UK Medical Research Council [FC001107]
- Wellcome Trust [FC001107]
- National Institute of Health [R01HD34283, R01HD30428]
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In the last 15 years, single-cell technologies have become robust and indispensable tools to investigate cell heterogeneity. Beyond transcriptomic, genomic and epigenome analyses, technologies are constantly evolving, in particular toward multi-omits, where analyses of different source materials from a single cell are combined, and spatial transcriptomics, where resolution of cellular heterogeneity can be detected in situ. While some of these techniques are still being optimized, single-cell RNAseq has commonly been used because the examination of transcriptomes allows characterization of cell identity and, therefore, unravel previously uncharacterized diversity within cell populations. Most endocrine organs have now been investigated using this technique, and this has given new insights into organ embryonic development, characterization of rare cell types, and disease mechanisms. Here, we highlight recent studies, particularly on the hypothalamus and pituitary, and examine recent findings on the pancreas and reproductive organs where many single-cell experiments have been performed.
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