Discrimination of common isomerides of methyl nucleosides by collision-induced dissociation tandem mass spectrometry

The methyl-modified nucleosides (methyladenosine, methylguanosine, methylcytidine, and methyluridine) occur widely in nucleic acids and serve as biomarkers for a variety of types of diseases. Their isomers have the same parent ions in MS spectra and need further discrimination. Here, electrospray ionization mass spectrometry (ESI-MS) coupled with collision induced dissociation (CID) was used to distinguish the common isomerides of methyl-nucleosides. The various fragmentation patterns had been discussed in comparison of the different methyl modified nucleosides and were studied as a function of normalized collision energy. Then, structurally relevant fragments were obtained to efficiently identify characterization of methyl-nucleoside isomerides by collision induced dissociation. Therefore, this study provides a promising method using CID-MS for the discrimination of the isomeric methyl nucleosides, which could be useful to quantitative study of methyl nucleosides and detect of unknown methyl nucleosides.

Automated summary

In this study, ESI-MS coupled with CID was used to distinguish the isomeric methyl nucleosides by analyzing their fragmentation patterns and molecular structures. Structurally relevant fragments were obtained to efficiently identify the isomerides, providing a promising method for quantitative study and detection of unknown methyl nucleosides.