Journal
JOURNAL OF INVERTEBRATE PATHOLOGY
Volume 173, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jip.2020.107367
Keywords
Decapod iridescent virus 1; Shrimp hemocyte iridescent virus; Cherax quadricarinatus iridovirus; Real-time PCR; Shrimp
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Funding
- Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao) [2018SDKJ0502-3]
- National Key R&D Program of China [2019YFD0900101]
- China Postdoctoral Science Foundation [2019M650170]
- Postdoctoral innovation project of Shandong Province [201902043]
- Postdoctoral Applied Research Foundation of Qingdao
- China Agriculture Research System [CARS-47]
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Decapod iridescent virus 1 (DIV1) was proven to be the aetiological agent of a disease causing mass die-offs of shrimp, prawn and crayfish. The specific purpose of this study was to develop a new sensitive real-time PCR method for the specific detection of DIV1. A pair of primers that amplify a 142 bp fragment and a TaqMan probe were selected for the major capsid protein gene of DIV1. They were shown to be specific for DIV1 and did not react with other common shrimp pathogens or healthy shrimp DNA. The method could detect as virus levels as low as 1.2 copies of DIV1 plasmid DNA.
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