Journal
JOURNAL OF EUKARYOTIC MICROBIOLOGY
Volume 67, Issue 5, Pages 612-622Publisher
WILEY
DOI: 10.1111/jeu.12813
Keywords
Biodiversity; biogeography; biological interactions; ecological patterns; function; phylogenetics
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Funding
- International Society of Protistologists
- Gordon and Betty Moore Foundation
- US National Science Foundation [OCE1924527]
- Science for Life Laboratory (SciLifeLab)
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During the last decade, high-throughput metabarcoding became routine for analyzing protistan diversity and distributions in nature. Amid a multitude of exciting findings, scientists have also identified and addressed technical and biological limitations, although problems still exist for inference of meaningful taxonomic and ecological knowledge based on shortDNAsequences. Given the extensive use of this approach, it is critical to settle our understanding on its strengths and weaknesses and to synthesize up-to-date methodological and conceptual trends. This article summarizes key scientific and technical findings, and identifies current and future directions in protist research that uses metabarcoding.
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