Journal
JOURNAL OF CLINICAL VIROLOGY
Volume 129, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jcv.2020.104521
Keywords
COVID-19; SARS-CoV2; SARS; Luminex; Serology
Categories
Funding
- Institut de Recherche pour le Developpement (IRD)
- French Agence Nationale de la Recherche (ANR
- ZOOCOV grant)
- Montpellier University of Excellence (MUSE) emergency response funding (PANCOV-S grant)
- INSERM
- University of Montpellier
- IRD
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Background: Knowledge of the COVID-19 epidemic extent and the level of herd immunity is urgently needed to help manage this pandemic. Methods: We used a panel of 167 samples (77 pre-epidemic and 90 COVID-19 seroconverters) and SARS-CoV1, SARS-CoV2 and MERS-CoV Spike and/or Nucleopcapsid (NC) proteins to develop a high throughput multiplex screening assay to detect IgG antibodies in human plasma. Assay performances were determined by ROC curves analysis. A subset of the COVID-19+ samples (n= 36) were also tested by a commercial NC-based ELISA test and the results compared with those of the novel assay. Results: On samples collected >= 14 days after symptoms onset, the accuracy of the assay is 100 % (95 % CI: 100-100) for the Spike antigen and 99.9 % (95 % CI:99.7-100) for NC. By logistic regression, we estimated that 50 % of the patients have seroconverted at 5.7 +/- 1.6; 5.7 +/- 1.8 and 7.9 +/- 1.0 days after symptoms onset against Spike, NC or both antigens, respectively and all have seroconverted two weeks after symptoms onset. IgG titration in a subset of samples showed that early phase samples present lower IgG titers than those from later phase. IgG to SARS-CoV2 NC cross-reacted at 100 % with SARS-CoV1 NC. Twenty-nine of the 36 (80.5 %) samples tested were positive by the commercial ELISA while 31/36 (86.1 %) were positive by the novel assay. Conclusions: Our assay is highly sensitive and specific for the detection of IgG antibodies to SARS-CoV2 proteins, suitable for high throughput epidemiological surveys. The novel assay is more sensitive than a commercial ELISA.
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