Journal
JOURNAL OF CHROMATOGRAPHY A
Volume 1620, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.chroma.2020.461036
Keywords
Camptotheca acuminate; In-house database; Untapped alkaloid; Biosynthetic precursor
Funding
- National Natural Science Foundation of China [21708028]
- Education Department of Sichuan Province, PR China [17ZA0301]
- National College Students Innovation and Entrepreneurship Training Program, PR China [201910626009]
- Scientific Innovation Cultivation Project of Sichuan Province, PR China [2018080]
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Leaves, flowers, fruits and stems (44 sample groups) were collected from mature Camptotheca acuminate during 2017.3-2018.3 and classified by ultra-high performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry based metabolomics. One hundred metabolites including forty-seven alkaloids, fifteen terpenes, thirty-two polyphenols and six other metabolites were rapidly identified through the in-house database alignment at first glance. Thirty-three alkaloids classified into five groups including camptothecin group (CG1-13), pumiloside group (PG1-5), strictosidinic acid group (SG1-3), vincosamide group (VG1-7), and a new hybrid group, vincosamide-camptothecin group (VC1-5) were mined and further characterized by MS/MS analyses. The identification of two untapped biosynthetic precursors, 2-hydroxypumiloside (PG2) and 16-hydroxy-15, 16-dihydrocamptothecoside (CG3), along with sixteen new alkaloids enables us for a better understanding of camptothecin biogenetic reasoning. The underlying enzymes involved in camptothecin biosynthesis were also proposed according to the guiding metabolic map, thus purposefully mining of enzymes involved in the downstream biosynthetic pathway of camptothecin could be initiated with the help of this map. (C) 2020 Elsevier B.V. All rights reserved.
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