Journal
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
Volume 40, Issue 10, Pages 1975-1986Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/0271678X20924954
Keywords
Glymphatic system; cerebrospinal fluid; anaesthesia; optical tissue clearing; light sheet microscopy
Categories
Funding
- Knut and Alice Wallenberg Foundation
- Torsten Soderberg Foundation
- Crafoord Foundation
- Swedish Research Council
Ask authors/readers for more resources
Fluid transport in the perivascular space by the glia-lymphatic (glymphatic) system is important for the removal of solutes from the brain parenchyma, including peptides such as amyloid-beta which are implicated in the pathogenesis of Alzheimer's disease. The glymphatic system is highly active in the sleep state and under the influence of certain of anaesthetics, while it is suppressed in the awake state and by other anaesthetics. Here we investigated whether light sheet fluorescence microscopy of whole optically cleared murine brains was capable of detecting glymphatic differences in sleep- and awake-mimicking anaesthesia, respectively. Using light-sheet imaging of whole brains, we found anaesthetic-dependent cerebrospinal fluid (CSF) influx differences, including reduced tracer influx along tertiary branches of the middle cerebral artery and reduced influx along dorsal and anterior penetrating arterioles, in the awake-mimicking anaesthesia. This study establishes that light sheet microscopy of optically cleared brains is feasible for quantitative analyses and can provide images of the entire glymphatic system in whole brains.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available