Journal
JOURNAL OF BIOLOGICAL CHEMISTRY
Volume 295, Issue 32, Pages 11118-11130Publisher
ELSEVIER
DOI: 10.1074/jbc.RA120.014010
Keywords
peroxiredoxin; thioredoxin; thiol; oxidative stress; redox regulation; hydroperoxide; alkyl hydroperoxide reductase; peroxiredoxin
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Funding
- National Natural Science Foundation of China [31930003, 41976087]
- Natural Science Foundation of Zhejiang Province [LZ17C010001]
- Ten Thousand Talent Program of China
- Fundamental Research Funds for the Central Universities
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AhpC is a bacterial representative of 2-Cys peroxiredoxins (Prxs) with broad substrate specificity and functional plasticity. However, details underpinning these two important attributes of AhpC remain unclear. Here, we studied the functions and mechanisms of regulation of AhpC in the facultative Gram-negative anaerobic bacteriumShewanella oneidensis, in which AhpC's physiological roles can be conveniently assessed through its suppression of a plating defect due to the genetic loss of a major catalase. We show that successful suppression can be achieved only when AhpC is produced in a dose- and time-dependent manner through a complex mechanism involving activation of the transcriptional regulator OxyR, transcription attenuation, and translation reduction. By analyzing AhpC truncation variants, we demonstrate that reactivity with organic peroxides (OPs) rather than H(2)O(2)is resilient to mutagenesis, implying that OP reduction is the core catalytic function of AhpC. Intact AhpC could be recycled only by its cognate reductase AhpF, and AhpC variants lacking the Prx domain or the extreme C-terminal five residues became promiscuous electron acceptors from the thioredoxin reductase TrxR and the GSH reductase Gor in addition to AhpF, implicating an additional dimension to functional plasticity of AhpC. Finally, we show that the activity ofS. oneidensisAhpC is less affected by mutations than that of itsEscherichia colicounterpart. These findings suggest that the physiological roles of bacterial AhpCs are adapted to different oxidative challenges, depending on the organism, and that its functional plasticity is even more extensive than previously reported.
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