Journal
BMC BIOINFORMATICS
Volume 16, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12859-015-0703-0
Keywords
DNA fingerprinting; Image analysis; Clustering; Java; Database
Categories
Funding
- Agencia de Desarrollo Economico de La Rioja (ADER) [2013-I-IDD-00123]
- Ministerio de Economia y Competitividad [MTM2014-54151-P]
Ask authors/readers for more resources
Background: DNA fingerprinting is a technique for comparing DNA patterns that has applications in a wide variety of contexts. Several commercial and freely-available tools can be used to analyze DNA fingerprint gel images; however, commercial tools are expensive and usually difficult to use; and, free tools support the basic functionality for DNA fingerprint analysis, but lack some instrumental features to obtain accurate results. Results: In this paper, we present GelJ, a feather-weight, user-friendly, platform-independent, open-source and free tool for analyzing DNA fingerprint gel images. Some of the outstanding features of GelJ are mechanisms for accurate lane- and band-detection, several options for computing migration models, a number of band- and curve-based similarity methods, different techniques for generating dendrograms, comparison of banding patterns from different experiments, and database support. Conclusions: GelJ is an easy to use tool for analyzing DNA fingerprint gel images. It combines the best characteristics of both free and commercial tools: GelJ is light and simple to use (as free programs), but it also includes the necessary features to obtain precise results (as commercial programs). In addition, GelJ incorporates new functionality that is not supported by any other tool.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available