Expression, Purification, and Evaluation of Anti-IL-2Rα Antibody Secreted by Leishmania tarentolae

Producing a functional anti-IL-2R alpha antibody inLeishmania tarentolae, a trypanosomatid protozoan non-pathogenic to human, is a cost-effective and safe alternative for the production of therapeutic recombinant proteins. Expression cassettes encoding heavy and light chains of the anti-IL-2R alpha antibody were cloned into two separate pLEXSY vectors. The plasmids were then used to transfectL. tarentolaelaboratory strain p10 and stable expression of a recombinant humanized anti-IL-2R alpha antibody was obtained. The heavy and light chains successfully assembled to produce a tetrameric 150 kDa antibody. The antibody was glycosylated and functional as assessed by enzymatic deglycosylation and cell proliferation assays respectively.L. tarentolaecan be used as an efficient, cost and labor-effective expression system for the production of therapeutic recombinant proteins.

Automated summary

Using Leishmania tarentolae as an expression system for producing therapeutic recombinant proteins is cost-effective, efficient, and requires less labor.