Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/ijms21165638
Keywords
deubiquitination assay; Ub-probes; activity-based probes; cell permeability
Funding
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT [2020R1A4A4079494, 2019R1A2C2004052, 2017R1A2B3007224]
- National Research Foundation of Korea [2019R1A2C2004052, 2020R1A4A4079494, 2017R1A2B3007224] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Deubiquitinating enzymes regulate various cellular processes, particularly protein degradation, localization, and protein-protein interactions. The dysregulation of deubiquitinating enzyme (DUB) activity has been linked to several diseases; however, the function of many DUBs has not been identified. Therefore, the development of methods to assess DUB activity is important to identify novel DUBs, characterize DUB selectivity, and profile dynamic DUB substrates. Here, we review various methods of evaluating DUB activity using cell lysates or purified DUBs, as well as the types of probes used in these methods. In addition, we introduce some techniques that can deliver DUB probes into the cells and cell-permeable activity-based probes to directly visualize and quantify DUB activity in live cells. This review could contribute to the development of DUB inhibitors by providing important information on the characteristics and applications of various probes used to evaluate and detect DUB activity in vitro and in vivo.
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