Journal
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Volume 21, Issue 15, Pages -Publisher
MDPI
DOI: 10.3390/ijms21155367
Keywords
photodynamic inactivation; porphyrins; photosensitisers; antibacterial activity; Legionella pneumophila; Acanthamoeba castellanii
Funding
- University of Rijeka [17.12.2.1.01, uniri-prirod-18-173, uniri-biomed-18-171]
- European Regional Development Fund for the Center of Excellence for Advanced Materials and Sensing Devices [KK.01.1.1.01.0001]
- ERDF funded project Research Infrastructure for Campus-based Laboratories at University of Rijeka
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Legionella pneumophilais an environmental bacterium, an opportunistic premise plumbing pathogen that causes the Legionnaires' disease.L. pneumophilapresents a serious health hazard in building water systems, due to its high resistance to standard water disinfection methods. Our aim was to study the use of photodynamic inactivation (PDI) againstLegionella. We investigated and compared the photobactericidal potential of five cationic dyes. We tested toluidine blue (TBO) and methylene blue (MB), and three 3-N-methylpyridylporphyrins, one tetra-cationic and two tri-cationic, one with a short (CH3) and the other with a long (C17H35) alkyl chain, againstL. pneumophilain tap water and after irradiation with violet light. All tested dyes demonstrated a certain dark toxicity againstL. pneumophila; porphyrins with lower minimal effective concentration (MEC) values than TBO and MB. Nanomolar MEC values, significantly lower than with TBO and MB, were obtained with all three porphyrins in PDI experiments, with amphiphilic porphyrin demonstrating the highest PDI activity. All tested dyes showed increasing PDI with longer irradiation (0-108 J/cm(2)), especially the two hydrophilic porphyrins. All three porphyrins caused significant changes in cell membrane permeability after irradiation andL. pneumophila, co-cultivated withAcanthamoeba castellaniiafter treatment with all three porphyrins and irradiation, did not recover in amoeba. We believe our results indicate the considerable potential of cationic porphyrins as effective anti-Legionellaagents.
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