Journal
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
Volume 95, Issue -, Pages 8-12Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijid.2020.03.055
Keywords
Broad-range polymerase chain reaction; Metagenomic next-generation sequencing; Prosthetic joint infection; Joint fluid
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Funding
- Foreign Cooperation Project of Fujian Natural Science Foundation [2019I0011]
- Industry Education Cooperation Project of Fujian Natural Science Foundation [2018Y4003]
- Fujian Education and Scientific Research Projects for Young Teachers [JAT170241]
- Startup Fund for scientific research, Fujian Medical University [2018QH1066]
- National Natural Science Foundation of China [81772251]
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Purpose: The aims of our study were to (1) evaluate the concordance of both methods for detecting prosthetic joint infection (PJI) pathogens in joint fluid and to (2) clarify whether broad-range polymerase chain reaction (BR-PCR) can be used as a verification method for metagenomic next-generation sequencing (mNGS) for PJI diagnosis. Methods: In total, 63 patients underwent total joint arthroplasty, with 45 PJI and 18 aseptic failure patients included. Joint fluids were sampled after antibiotics were withheld for more than 2 weeks, and then, culture, BR-PCR and mNGS were performed for all samples. Results: The joint fluid BR-PCR sensitivity was 82.2%, which was not significantly different from that of mNGS (95.6%) or culture (77.8%). The specificities of the 3 methods were all 94.4%. BR-PCR failed to identify the pathogens in 1 polymicrobial infection patient and 4 fungal infection patients. Conclusion: mNGS was more sensitive than BR-PCR for detecting PJI pathogens in joint fluid. BR-PCR is insufficient for use as an mNGS verification method. (C) 2020 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
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