Journal
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
Volume 15, Issue 8, Pages 7347-7358Publisher
ESG
DOI: 10.20964/2020.08.70
Keywords
DNA; Biosensor; Cadmium; Brilliant green; Rat
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Funding
- National Key Research and Development Program of China [2016YFD0501208]
- Project of the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Key Research and Development Program of Yangzhou City [YZ2017081]
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A highly sensitive electrochemical biosensor was fabricated for Cd2+ determination based on a dsDNA modified carbon paste electrode (CPE) and brilliant green (BG) indicator. The binding of Cd2+ on the modified dsDNA could result in the destabilization of the double helix structure of DNA, which was detected by the oxidation of a DNA hybridization indicator BG on the electrode surface. The reduction current of BG on the modified electrode increased significantly with the presence of Cd2+. Based on such electrochemical response, this electrode was further used determine the concentration of Cd2+ with a linear range from 0.05 x10(-9) to 1.2 x10(-9) mol/L and a limit of detection of 0.1 x10(-12) mol/L. The portable, low-cost modified electrode showed good sensitivity, selectivity, and stability. The method developed in this study was also applied to the direct determination of Cd2+ in rat tissue samples with satisfactory results. This work reveals that the dsDNA-modified CPE is a promising tool for the food detection and animal diagnosis.
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