Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 162, Issue -, Pages 1725-1733Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.003
Keywords
Antifungal; Antioxidant; Immunomodulation; Lignin
Funding
- Coordination for the Improvement of Higher Education Personnel (CAPES)
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Caesalpinia pulcherrima is a shrub with worldwide distribution used as an ornamental plant. In this study, we extracted a lignin from the C. pulcherrima leaves and investigated its biological functions. The lignin was characterized by FT-IR, UV-Vis, GPC, TGA and nuclear magnetic resonance (1H and C-13). The antioxidant activity was evaluated using phosphomolybdenum complexation methods (TAA), sequestration of DPPH and ABTS radicals, reducing power, formation of nitrite radical and iron chelating activity (Fe2+). Antifungal activity was made using Candida spp., Aspergillus spp. and Cryptococcus neoformans strains. Cytotoxicity, oxidative stress, and cytokine production were performed using mouse splenocytes. The lignin showed maximal UV-Vis at similar to 280 nm, 22.27 L/g.cm of absorptivity and, 2,503 kDa of molecular weight. Phenolic compounds (41.33 0.65 mg GAE/ g) and indications of a guaiacyl-syringyl-hydroxyphenyl (GSH)-type composition were found. Antioxidant activities of lignin to TAA (40 +/- 1.2%) and to DPPH (16.9 +/- 0.2%) was high and showed antifungal potential, especially against Candida spp. (IC50 = 31.3 Ng/mL) and C. neoformans (15.6 Ng/mL). In mouse splenocytes, the lignin was not cytotoxic and stimulated the cell proliferation and cytokine release. These results indicate that C. pulcherrima lignin has the potential to be used as antifungal and immunostimulant compound.(c) 2020 Elsevier B.V. All rights reserved.
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