Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 157, Issue -, Pages 510-521Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.04.120
Keywords
Sucrose synthase; Glycosyltransferase; Coimmobilization; Colocalization; Piceid; Glycobiotechnology
Funding
- EU FP7 project SuSy (Sucrose Synthase as Cost-Effective Mediator of Glycosylation Reactions) [C-KBBE/3293]
- Juan de la Cierva fellowship - Spanish Ministry of Economy, Industry and Competitiveness [IJCI-2014-19260]
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Glycosylation is one of the most efficient biocompatible methodologies to enhance the water solubility of natural products, and therefore their bioavailability. The excellent regio- and stereoselectivity of nucleotide sugar-dependent glycosyltransferases enables single-step glycosylations at specific positions of a broad variety of acceptor molecules without the requirement of protection/deprotection steps. However, the need for stoichiometric quantities of high-cost substrates, UDP-sugars. is a limiting factor for its use at an industrial scale. To overcome this challenge, here we report tailor-made coimmobilization and colocalization procedures to assemble a bienzymatic cascade composed of a glycosyltransferase and a sucrose synthase for the regioselective 5-O-beta-D-glycosylation of piceid with in situ cofactor regeneration. Coimmobilization and colocalization of enzymes was achieved by performing slow immobilization of both enzymes inside the porous support The colocalization of both enzymes within the porous structure of a solid support promoted an increase in the overall stability of the bi-enzymatic system and improved 50-fold the efficiency of piceid glycosylation compared with the non-colocalized biocatalyst. Finally, piceid conversion to resveratrol 3,5-diglucoside was over 90% after 6 cycles using the optimal biocatalyst and was reused in up to 10 batch reaction cyder accumulating a ITN of 91.7 for the UDP recycling. (C) 2020 Elsevier B.V. All rights reserved.
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