Blend biopolymeric nanofibrous scaffolds of cellulose acetate/ε-polycaprolactone containing metallic nanoparticles prepared by laser ablation for wound disinfection applications

Laser ablation technique was utilized to synthesize versatile metallic nanoparticles including ZnO, Ag and CuO which were incorporated into the blend matrix of cellulose Acetate (CA) and epsilon-polycaprolactone (PCL) nanofibrous scaffold. The compositional, microstructural and morphological behaviors for the obtained nanofibers were investigated using X-ray diffraction (XRD), Fourier Transformed Infrared, Transmission Electron Microscope (TEM) and Field Emission-Scanning Electron Microscope (FESEM). The cell viability and antibacterial activity were investigated against Staphylococcus aureus (S. aureus) and Escherichia coli (E-coli). TEM micrographs refer that while CuONPs were involved in the middle of CA/PCL fibrous scaffold with diameters around 160 nm. Themorphological investigations indicated the scaffolds were configured in a non-oriented form with diameters 0.45-0.9 mu m in the case of ZnONPs involved in blend matrix fibers. The ratio of viable cells displays that compositions are biocompatible, while the antibacterial activity of both AgNPs and CuONPs showed an inhibition zone around 11.2.3 +/- 2.2 mm and 9.4 +/- 1.2 mm respectively. Bio-blend polymers matrices carrying nanoparticles could be tailored for a plethora of biomedical applications upon their compositions. (C) 2020 Published by Elsevier B.V.