Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 164, Issue -, Pages 2092-2099Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.08.085
Keywords
Starch liquefaction; Fine regulation; Aspergillus niger
Funding
- Postdoctoral Science Foundation of Henan Province [1902043]
- High-level Talent Programof Henan Institute of Science and Technology [2017003]
- Key Laboratory of Viticulture and Enology of Ministry of Agriculture [KLVE201702]
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Citric acid (CA), generally fermented from the starchy material, has attracted intense attention because of its wide applications in many aspects. However, the traditional starchy-liquefaction process based on the dextrose equivalent value and iodine-testing method is inappropriate as it hinders the subsequent CA fermentations. Here, a novel method of evaluating the starch liquefaction in the CA production process was established. Firstly, dextrin samples with the molecular weight (M-w) narrow distribution were prepared by alcohol fractional precipitation. Glucoamylase (GM) from the culture of CA-producing strain Aspergillus niger was purified. Then, the structure-activity relationship between the dextrin and GM was analyzed. Results demonstrated that the M-w of liquefied components aggregated in the range of 1.4-1.9 kDa could improve the efficiency of simultaneous saccharification and fermentation process. CA production rate and total sugar uptake rate were evidently improved with residual total sugar decreasing by 10.8% and fermentation efficiency enhanced by 21.1% in 9 h shorter fermentation time. All these results confirmed that fine regulation of the starch liquefaction based on the M-w characteristics was feasible and effective to improve the CA fermentation. Our proposed strategy could also be useful for other fermentations and sugar industry involving the starchy material. (C) 2020 Elsevier B.V. All rights reserved.
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