Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 152, Issue -, Pages 846-861Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.02.245
Keywords
Chitosan; Schiff base; Cytotoxicity; Flow cytometry; MTT assay
Funding
- Semnan University of Medical Sciences and Semnan University, Iran
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Chitosan derivatives are widely used as key classes of medicinal compounds owing to their non- toxic and biodegradable properties. So, in this work, to enhance chitosan biological activities, a new synthesis of a series of Schiff base and its metals complexes (Cu(II), Ni(II) and Zn(II)) of chitosan (CS) was prepared. Moreover, their physicochemical properties were characterized by IR, UV-Vis, SEM, melting point, thermo gravimetric analysis (TGA), X-ray diffraction (XRD), elemental analysis and H-1 NMR techniques. Elemental analysis data confirmed the formation of chitosan-Schiff base as well as the coordination reaction with metals ions by increasing the carbon content caused by substitution. By elemental analysis, the degrees of acetylation (DA), deacetylation (DD) and substitution (DS) were acquired 23, 77.63 and 57.90%, respectively. Additionally, the 'H NMR spectroscopy was used for the determination of degree of deacetylation (DD) and Substitution (DS) of chitosan ranging from 87.5 and 85%, respectively. The presence of a new low-field signal at 1023 ppm in the H-1 NMR spectra confirmed the imine proton of Schiff base. The cytotoxicity of Chitosan, Chitosan-Schiff base and its metals complexes was tested against K562 chronic myelogenous leukemia (CML) and MG-63 (osteosarcoma cancer) cell lines by the MTT assay. The results suggested that the anticancer activity of Schiff base and their complexes was much better than that of pure CS against cancer MG63 cell line. Finally, through flow cytometty, we demonstrated that all compounds were efficient in inducing apoptosis effect in K562 and MG63 cell lines except Schiff base- chitosan in K562 cell lines. (C) 2020 Published by Elsevier B.V.
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