Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 154, Issue -, Pages 751-757Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2020.03.154
Keywords
Glucose generation rate; Mammalian glucosidases; Carbohydrate digestion
Funding
- Nestle Research Project - Nestle Research at Lausanne, Switzerland [DUND-101451]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2018R1D1A1B07047650]
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The supernatant from rat intestinal acetone powder (RIAP) was used as a source of mammalian glucosidases to determine the digestion properties of glycemic-carbohydrates. We hypothesized that many glucosidases are still anchored to the precipitated-intestinal tissues with available enzymes, and developed a method using the RIAP suspension to optimize the in vitro carbohydrate digestion model. The glucose production from various types of glycemic ingredients by RIAP suspension showed that this carbohydrate-hydrolysis model using the entire spectrum of glucosidases can be applied in an in vitro assay to determine carbohydrate quality from glycemic food products at the mammalian level. This approach better mimics the mammalian situation compared to other assays to determine the glycemic-carbohydrate digestion properties that employ fungal/microbial glucosidases that have different hydrolytic activities compared to mammalian enzymes. The method can also be used to determine the inhibitory effects of alpha-glucosidase inhibitors to attenuate the post-prandial blood glucose level. (C) 2020 Elsevier B.V. All rights reserved.
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