Journal
IMMUNOLOGY AND CELL BIOLOGY
Volume 98, Issue 7, Pages 528-539Publisher
WILEY
DOI: 10.1111/imcb.12363
Keywords
Dynamin-related protein 1; lipopolysaccharide; macrophage; mitochondrial dynamics; mitochondrial fission; Toll-like receptor
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Funding
- National Health and Medical Research Council of Australia (NHMRC) [APP1125316]
- NHMRC [APP1160106, APP1107914, APP1117017, APP1141131]
- Rebecca Cooper Foundation [PG2018095]
- Australian Research Council Centre of Excellence in Advanced Molecular Imaging [CE140100011]
- Australian Research Council [DE1310470]
- NL K.F. Hein Fonds [1652430]
- Institute for Molecular Bioscience Dynamic Imaging Facility for Cancer Biology at The University of Queensland
- Australian Cancer Research Foundation
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Mitochondria have a multitude of functions, including energy generation and cell signaling. Recent evidence suggests that mitochondrial dynamics (i.e. the balance between mitochondrial fission and fusion) also regulate immune functions. Here, we reveal that lipopolysaccharide (LPS) stimulation increases mitochondrial numbers in mouse bone marrow-derived macrophages (BMMs) and human monocyte-derived macrophages. In BMMs, this response requires Toll-like receptor 4 (Tlr4) and the TLR adaptor protein myeloid differentiation primary response 88 (MyD88) but is independent of mitochondrial biogenesis. Consistent with this phenomenon being a consequence of mitochondrial fission, the dynamin-related protein 1 (Drp1) GTPase that promotes mitochondrial fission is enriched on mitochondria in LPS-activated macrophages and is required for the LPS-mediated increase in mitochondrial numbers in both BMMs and mouse embryonic fibroblasts. Pharmacological agents that skew toward mitochondrial fusion also abrogated this response. LPS triggered acute Drp1 phosphorylation at serine 635 (S635), followed by sustained Drp1 dephosphorylation at serine 656 (S656), in BMMs. LPS-induced S656 dephosphorylation was abrogated inMyD88-deficient BMMs, suggesting that this post-translational modification is particularly important for Tlr4-inducible fission. Pharmacological or genetic targeting of Tlr4-inducible fission had selective effects on inflammatory mediator production, with LPS-inducible mitochondrial fission promoting the expression and/or secretion of a subset of inflammatory mediators in BMMs and mouse embryonic fibroblasts. Thus, triggering of Tlr4 results in MyD88-dependent activation of Drp1, leading to inducible mitochondrial fission and subsequent inflammatory responses in macrophages.
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