Journal
FOOD RESEARCH INTERNATIONAL
Volume 134, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2020.109223
Keywords
Espresso coffee; Single-dose capsule; Phenolic compounds; Chlorogenic acids; Antioxidant activity; Gastrointestinal digestion
Categories
Funding
- Fundo Europeu de Desenvolvimento Regional (FEDER) through the Programa Operacional Competitividade e Internacionalizacao (POCI) under the ESSENCE Project [POCI-01-0247-FEDER-010887]
- CBQF under the FCT [UID/Multi/50016/2019]
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The single-dose coffee capsule is the most successful technology used to prepare espresso coffee (EC). However, the characterization of ECs extracted using this technology, regarding chlorogenic acids (CGAs) composition, antioxidant activity and stability during gastrointestinal digestion (GID), are still limited. The aim of this research work was: (i) to characterize the phenolic profile and antioxidant activity of 11 commercial ECs from single-dose capsule system and (ii) to evaluate the impact of the in vitro GID on the stability of CGAs. Within all the ECs analysed it was detected the presence of 4 caffeoylquinic acids (CQAs), 1 feruloylquinic acids, 2 caffeoylshikimic acids and 3 diCaffeoylquinic acids (di-CQAs). The major compound in all ECs was 5-CQA, followed by 4-CQA and 3-CQA. The di-CQAs were found in lower concentration than CQAs (4,5-diCQA > 3,4-diCQA > 3,5-diCQA). The total CQAs and diCQAs content of the ECs analyzed ranged from 1.86 +/- 0.19 to 2.42 +/- 0.28 and 0.26 +/- 0.02 to 0.42 +/- 0.06 mg/mL of EC, respectively. The high antioxidant activity of the ECs is related with the high CGAs concentration, which ranged from 4.92 +/- 0.29 to 7.28 +/- 0.25 mg AAE/mL of EC and from 6.13 +/- 0.37 to 10.07 +/- 0.17 mg TE/mL of EC for ABTS degrees and DPPH degrees methods, respectively. The principal component analysis showed that the coffee variety used in ECs preparation explained 74.8% of the results' variation and that 6 of the total number of ECs were related with high CGAs contents and antioxidant activity. The GID induced a decrease in most CGAs, which had a direct impact on the antioxidant activity, therefore concerning EC ingestion the CGAs bioaccessible concentration decreased in comparison with a non-digested EC. Nevertheless, the CGAs concentration available after GID is still sufficiently high to exert antioxidant activity (measured in vitro) that may exert a potential beneficial effect on humans health.
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